The manual microscope stitching system is compatible with conventional manual XY stage microscopes, eliminating the need for electric scanning hardware. Operators manually translate the stage and sequentially capture multiple frames of microscopic images with overlapping areas. The system relies on feature matching algorithms to complete image registration, coordinate correction, and fusion processing, automatically synthesizing scattered small fields of view into a large-scale, high-resolution panoramic microscopic image. The system supports image stitching for brightfield, stained smear, and microscopic pathogen samples, effectively solving the problem of limited field of view with high-power objectives and preserving the global morphology and local details of microscopic samples. Addressing the characteristics of weak texture and uneven illumination in microscopic images, the system incorporates robust feature matching and multi-band image fusion strategies to eliminate stitching gaps, balance brightness and darkness differences in the image, and output panoramic digital slide images that can be directly used for observation, annotation, target detection, and archiving.